The diagnosis of cirrhosis was based on the clinical and laboratory signs of hepatocellular failure and portal hypertension such as stellar angioma, encephalopathy, low serum albumin level, prolonged prothrombin time, ascites, jaundice, esophageal or gastric varices, and irregular hepatic echogenicity of the liver in abdominal US. This evidence concerns the gene F2 and Encephalopathy.