Tumor B-cells can also directly express a variety of ligands and suppressive cytokines such as a) TGF-β, which promotes Treg development and inhibits CD4+ TL differentiation into Th1 or Th17, or b) IL-10 which promotes CD4+ CD25+ FOXP3+ Tregs development and CD5+ B-cell expression of Fas-L, leading to cell death in vitro (Figure 6) (112). This evidence concerns the gene TGFB1 and neoplasm.