RAF1 and liver cancer: As expected, after QGY7703 and SK-Hep1 cells exposed to the 150 μg/mL extracts for 48h, protein levels of Ras/Raf/MEK/ERK observably decreased, while the phosphorylation of Raf, MEK and ERK were inhibited by G85 to a greater extent than by GLE and G65 (Figure 6), which was consistent with their inhibition of proliferation and induction of apoptosis of human liver cancer cells.