To contribute the mechanism of TRPM7’s role in glioma, our current results first decipher that TRPM7 is responsible for sustained Notch1 signaling activation, enhanced expression of GSC markers CD133 and ALDH1, and regulation of glioma stemness, which contribute to malignant glioma cell growth and invasion. The gene discussed is PROM1; the disease is malignant glioma.