To this end, the different glioma cell lines were transfected with either control siRNA (siCtrl) or TRPM7 siRNA (siTRPM7), followed by assaying the expressions of Notch1, Notch2, Notch3 and Notch 4 in each of the following cell lines: A172, U87MG, SNB19, and U373MG. Here, NOTCH4 is linked to central nervous system cancer.