In addition, we also examined the potential effect of CD13 in HCC invasion by Boyden chamber trans‐well assays and found that CD13 depletion led to decreased cell invasion in HCCLM3 and MHCC97H cells (both P < .001, Figure 1F and Figure S1D), whereas CD13 overexpression in Huh7‐ and HepG2 enhanced invasive potential, which is significantly compared with the control groups (both P < .01, Figure 1F and Figure S1D). The gene discussed is ANPEP; the disease is hepatocellular carcinoma.