Next, T24T (anti‐miR516a/shPHLPP2 #2) cells were stably transfected with Flag‐SMURF1 or a control plasmid (Figure 6F) to confirm the role of SMURF1 in miR‐516a‐mediated promotion of BC migration and invasion. The gene discussed is SMURF1; the disease is breast cancer.