As control, WT and WT+OF were all normal in H&E staining, [HBx,src] transgenic zebrafish overfed (DIO group) displayed hyperplasia (80%) and HCC (20%), in contrast to the zebrafish fed a NOR, which were 100% normal according to H&E staining (Figure 2A). This evidence concerns the gene SRC and hepatocellular carcinoma.