To assess the resistance generated by disruption of genes encoding translation initiation factors 4E, we compared the phenotypic responses on infection with TuMV‐GFP UK1 of three Arabidopsis genotypes: a wild‐type susceptible accession Columbia‐0, a line knocked out for eIF4E1 (eif4e1KO), resistant to ClYVV, and a line knocked out for eIFiso4E (eifiso4eKO), resistant to TuMV. This evidence concerns the gene EIF4E and infection.