These findings imply that a positive feed forward loop between Wnt/β-catenin and NOX4 signaling promotes EMT property in BEAS-2B cells exposed to silica stimulation, which thus emphasize the profibrogenic role of the crosstalk of Wnt and NOX-mediated ROS in lung epithelial cell injury and development of silicosis, which warrants further investigations. This evidence concerns the gene NOX4 and silicosis.