To further confirm that miR-326 and LASP1 mediated the function of hsa_circRNA_000166 during CRC development, we conducted codepletion of both siRNA-1 and miR-326 I or depletion of siRNA-1 while there was overexpression of LASP1 and inspected the role of miR-326 and LASP1 in the regulation of hsa_circRNA_000166 in CRC development. This evidence concerns the gene LASP1 and colorectal carcinoma.