Meanwhile, the high glucose incubation increases CpG (5′—cytosine—phosphate—guanine—3′) methylation and increases in 5mCpG levels in promoter regions and silences the expression of many key genes involved in PI3K, cAMP, insulin, insulin secretion, diabetic, and NAFLD signaling pathways, leading to lipid accumulation [43]. The gene discussed is INS; the disease is metabolic dysfunction-associated steatotic liver disease.