PITX2 and neoplasm: The methylation status of five tumor-suppressor gene promoters was assessed: ATM including four evaluated CpG sites (CpG 1–4), PITX2 (CpG 1–5), RASSF1 (CpG 1–3), PTEN (CpG 1–6), and TIMP3 (CpG 1–6) (Figure 8).