As metformin is considered to regulate AMPK and human insulin resistance, we treated the aak-2(ok524) and daf-18(ok480) mutants with metformin and found that metformin could significantly suppress the Q-cell divisions in aak-2(ok524) L1-arrested mutants (Figure 1D), while metformin had no effect on daf-18(ok480) mutants—Figure 1E. As there are two AMPK catalytic subunits (AAK-1 and AAK-2) in C. elegans, metformin might work via AAK-1. This evidence concerns the gene AAK1 and Insulin resistance.