The objectives are firstly to investigate the impact of fish oil and/or probiotic supplementation from early pregnancy onwards on the serum hsCRP concentration and serum and vaginal levels of IGFBP-1, phIGFBP-1, and MMP-8 and secondly to determine whether these low-grade inflammatory and metabolic markers could predict the diagnosis of GDM. The gene discussed is IGFBP1; the disease is gestational diabetes.