RELA and esophageal squamous cell carcinoma: After confirming that SFE inactivated the NFκB pathway and decreased the content of p65 in the nuclear, we can conclude that SFE prevents p65 from functioning as a regulator of gene expression and resulting in the down-regulation of TNFAIP3 and PLAU. The dose-dependent inhibition of SFE on ESCC cell proliferation, invasion and migration had been confirmed previously [9], and data shown in Figure 5 could also indicate that SFE induced G2/M cell cycle arrest, cell apoptosis, and decreased the metastasis of ESCC cells.