The top 3 enriched pathways of these four genes in the ESCC samples are shown in Figure 3C, which shows a significant association between TNFα signaling via NFκB and high levels of TNFAIP3, INHBA, and PLAU. Although TNFα signaling via NFκB was not in the top 3 CXCL10-related pathways, it was statistically significant with NES >1.5. The gene discussed is TNFAIP3; the disease is esophageal squamous cell carcinoma.