However, when overexpressing TNFAIP3 and PLAU (Supplementary Figure S9A,B) or upon activation of the NFκB pathway, all the inhibitory effects of SFE were reversed (Figure 5A–D), suggesting that SFE blocked the p65 promotion of TNFAIP3 and PLAU expression to inhibit ESCC cell proliferation and metastasis. This evidence concerns the gene NFKB1 and esophageal squamous cell carcinoma.