The JASPAR database provides the sequence logo of p65 (Figure 4A) and predicts the binding sites in CXCL10, TNFAIP3, INHBA, and PLAU, and since TNFAIP3 and PLAU were the most down-regulated genes in the SFE-treated ESCC cells, we chose these genes for follow-up verification (Figure 4B). The gene discussed is CXCL10; the disease is esophageal squamous cell carcinoma.