We tried to use the RNA-seq data of 182 ESCC samples to further assess the biological characteristics of CXCL10, TNFAIP3, INHBA, and PLAU. The dataset was divided into a high-level group and a low-level group based on the median expression level of these 4 genes, and as shown in Supplementary Figure S4, all the high-level groups correlated with advanced pathology T stage and pathologic stage (except for TNFAIP3). The gene discussed is TNFAIP3; the disease is esophageal squamous cell carcinoma.