In vivo, ML/CT-2A tumors had lower numbers of CD45lowCD11blowCX3CR1+ microglial cells (considered activated or resting based on MHCII positive or negative staining, respectively), but higher numbers of CD11b+F4/80+CD64+Ly6C− resident macrophages and CD39+Tim3+Lag3+CD8+ exhausted cytotoxic T cells compared to other glioblastoma tumor models. This evidence concerns the gene LAG3 and neoplasm.