Western blot assay and immunofluorescence staining also demonstrated that DOX induces cardiac hypertrophy through GATA-4 and calcineurin/NFAT-3 signaling pathway, while pretreatment of BA reduced the p-GATA-4 and NFAT translocation as well as inhibited the calcineurin expression in DOX-induced H9c2 cells. This evidence concerns the gene CFB and cardiac hypertrophy.