Although it has been reported that M2 macrophages may promote EMT partially by increasing the secretion of cytokines and activating the AKT3/PRAS40 signaling pathway in ICC in vitro [34], the molecular mechanisms of ICC malignancy are not completely clear, and with the lack of clinical evidence, very little is known regarding their role in ICC in vivo. Here, AKT1S1 is linked to intrahepatic cholangiocarcinoma.