GLI2 and intrahepatic cholangiocarcinoma: ELISA confirmed a significant increase in IL-10 in M2/HuCCT1 or Huh28 coculture supernatants (553.5 pg/ml, 494.8 pg/ml) compared to HuCCT1 (150.9 pg/ml), Huh28 (297.07 pg/ml), THP-1/HuCCT1 or Huh28 (286.7 pg/ml, 330.63 pg/ml), THP-1 (142.9 pg/ml) and M2 macrophage (281.9 pg/ml) supernatants (Fig. 5b), indicating that IL-10 was involved in the ICC induction effects.