BCR and cancer: Given that the UHR sample is a mixture of 10 different cancer cell lines, it is expected that known gene fusions such as BCR-ABL1 in one cell line will have relatively low allele fraction in the data; therefore, in the long-read RNA-seq data generated by us, we do not have enough coverage on the BCR-ABL1 gene fusion and we were not able to detect this fusion by LongGF.