Next, the most active derivatives were studied in MCF-7 (breast adenocarcinoma, Ras/RAF wild type, and PIK3CA mutant), HeLa (cervical carcinoma, Ras/RAF and PIK3CA wild type), as well as HCT-116 and HKH-2 (colorectal carcinoma, KRas mutant and mutated KRas disrupted, respectively) cell lines [8]. The gene discussed is RAF1; the disease is colorectal carcinoma.