TMEFF2 and Familial prostate cancer: In both the androgen-dependent 22Rv1 prostate cancer cells and the androgen-independent PC3 cells, the removal of all four uORFs enhanced the translation of TMEFF2 mRNA; however, when DHT was added to the 22Rv1 cell culture, a concentration-dependent increase in luciferase activity was only observed in the construct harbouring all four uORFs.