To confirm that peripheral blood mMDSCs from CRC patients and healthy donors suppressed T-cell activation, we isolated mMDSCs, CD14− cells, and pan-T cells from PBMCs and co-cultured them under stimulation with anti-CD2/anti-CD3/anti-CD28 antibody conjugated beads for 5 d. Here, CD2 is linked to colorectal carcinoma.