For mMDSC titration assay (CRC patients: n = 3, healthy donors: n = 3), when autologous mMDSCs were added in a mMDSC:CD14− cell ratio of 0.25:1, 0.5:1 and 1:1, IFN-γ production of CD14− cells was inhibited by mMDSCs at a ratio of 1:1 and 0.5:1, and the loss of IFN-γ suppressive activity was observed as mMDSCs were titrated down in both CRC patients and healthy donors (Fig 4A). Here, IFNG is linked to colorectal carcinoma.