USP22 and colon carcinoma: To this end, USP22 expression was reduced using siRNA in the HT‐29 human colon carcinoma cell line, followed by induction of TNFα (T)/Smac mimetic (BV6; B)/zVAD.fmk (Z) (TBZ)‐mediated necroptosis and quantification of propidium iodide (PI) uptake, as marker for cell death.