The regulatory effect analysis of methylation profile in CRC shows a positive correlation between methylation in 5pUTR of RBM47, DGKA, ALKBH7 and TRAK1, and YAP1 protein level, negative correlation between LOC100130987 methylation and CTNNB1 protein level and positive correlation of TMEM156 methylation with FN1 protein level (Figure 6c and supplementary file 8). The gene discussed is TRAK1; the disease is colorectal carcinoma.