Then the expression of miR-4731, EGFR, ERK-1,2 and AKT-1,2 in two cell lines of glioblastoma (U-87MG and U-251MG) were evaluated by real-time PCR and compared with the glioblastoma cells transduced with the scramble vector as the control. This evidence concerns the gene AKT1 and glioblastoma.