Therefore, treatment with celastrol inhibited colorectal cancer cell growth and migration, and was associated with suppression of the expression of key genes [TYMP, CDH5, THBS2, LEP, MMP9, and tumor necrosis factor α (TNF)] and proteins (IL-1b, MMP-9, PDGF, Serpin E1, and TIMP-4) involved in the angiogenesis pathway. This evidence concerns the gene TNF and colorectal cancer.