To further explore the effect of SLC12A8 on the proliferation and motility of bladder cancer cells, CCK8 and colony formation assays were carried out in T24 and 5673 cells, which transfected with si-SLC12A8#1 and si-SLC12A8#2 or pcDNA3.1-SLC12A8, respectively. The gene discussed is SLC12A8; the disease is urinary bladder carcinoma.