Immunofluorescence microscopy revealed that non-infected Caco-2 monolayers are characterized by the uniform distribution of claudin-8 in the tight junction areas (Figure 1A), while infection with wt C. jejuni or the wt complemented strain (ΔhtrA/htrAwt) led to mislocalization of claudin-8 out of the tight junctions to form agglomerates in the cytoplasm (Figures 1B, E, white arrows). The gene discussed is CLDN8; the disease is infection.