Our study provides evidence that under hypoxic conditions CK inhibited the expression of Bclaf1 in Bel-7404 and Huh7 cells, promoted the degradation of HIF-1α ubiquitination, and inhibited the HIF-1α-mediated cellular glycolysis pathway to inhibit the proliferation of liver cancer cells in vitro. The gene discussed is HIF1A; the disease is liver cancer.