RT-qPCR was conducted to determine the expression of M1 markers II1b, Cd86, and Nos2 as well as M2 markers Cd163, Ym1, and Mrc1, displaying that infection with Lenti-EZH2 in glioma cells induced potent rise in the expression of II1b, Cd86, and Nos2, and marked reduction in the expression of Cd163, Ym1, and Mrc1 in macrophages (Figure 3F). Here, CD86 is linked to central nervous system cancer.