Similarly, the mutational upregulation of the Wnt pathway is seen in three out of the four HCC lines, with pathogenic activating mutations in CTNNB1 [S37C in SNU398 and in-frame deletion of amino acids 25–140 in HepG2 (27)] or loss-of-function mutation in Axin1 in Hep3B cells (Table 1 and Supplementary Figure 1). The gene discussed is CTNNB1; the disease is hepatocellular carcinoma.