Certain cancer lines (SW620, HT29, SW48, LS174T, SNU398, HepG2), already having high levels of the basal Wnt activation through mutations in the Wnt pathway components, did not reveal a strong stimulation of the pathway neither by Wnt3a nor by CHIR99021 (actually a decrease in the Wnt signaling levels can be induced e.g. in HT29 or HepG2). The gene discussed is WNT3A; the disease is cancer.