As expected (11), a general mutational portrait of Wnt pathway activation is detected for CRC lines with the gain-of-function mutations in CTNNB1 (encoding β-catenin) noticeable in LS174T and SW48 cells (S45F and S33Y, respectively), along with loss-of-function mutations in APC in SW620 and HT29 lines (homozygous truncations in the protein). This evidence concerns the gene APC and colorectal carcinoma.