We further performed immunofluorescence (IF) against MBNL1 in normal, untreated DM1, non-targeting and CUG-targeting DM1 myoblast cells (Supplementary Figure 5) and observed a more dispersed MBNL1 distribution in LshCas13a treated cells (Supplementary Figure 5), which may account for the splicing rescue. This evidence concerns the gene MBNL1 and myotonic dystrophy type 1.