Among these validated 25 variants, 15 variants with a frequency more than 3.64% (2/55), which consisted of MRPS5 rs200730619, XRRA1 rs180810179, KRT39 rs17843022, HIPK4 rs192823203, TACC2 rs140280635, FSIP2 rs181404625, FSIP2 rs188160736, PRRC2B rs201654931, SELO rs202018920, FRMD8 rs117981233, KLHL33 rs12587478, ADCY7 rs139279676, LRIG1 rs749143043, TEKT1 rs771448702 and KLHL35 rs774288909, were eventually determined for validation in additional 307 leprosy cases and 258 controls (S3 and S8 Tables). This evidence concerns the gene HIPK4 and leprosy.