Interestingly, higher tumor grades, i.e. a poor tumor differentiation, were associated with significantly lower AChE IHC scores (G1: 1.5 ± 0.2, G2: 1.1 ± 0.3, G3: 1.0 ± 0.4, Fig. 5g), suggesting a spontaneous loss of AChE in increasingly aggressive PCa. Here, ACHE is linked to neoplasm.