Expression of GLUT1, HK2, PKM2, LDHA, MCT4, and glycolytic regulators c-Myc, HIF-1α, p53, Akt, and SIRT6 was detected using western blot assays to determine the inhibitory mechanism of α-hederin on NSCLC A549 cells. This evidence concerns the gene HIF1A and non-small cell lung carcinoma.