RNA expression of genes related to proliferation (MKI67, CCNA2, CCNB1, and CCNB2), differentiation (EPCAM, CK8, and CK18), epithelial‐to‐mesenchymal transition (EMT) (SNAI1, FOSL1, and ID1), cancer stemness (CD44 and MALAT1), and pluripotency (POU5F1 and NANOG) were quantified in 2D‐grown cells and cells grown on colorectal PDS. Here, POU5F1 is linked to cancer.