However, at 3 h post infection, there were fewer ΔinvA mutant bacteria in the cytosol as compared to SPtA 9150 WT or ΔfepE, suggesting that vacuolar escape later during infection is dependent on the SPI‐1 T3SS but is not affected by the very long O‐antigen chains (Figure S5B). This evidence concerns the gene SPI1 and infection.