TNF and obesity due to melanocortin 4 receptor deficiency: The RT-PCR results of wound tissue showed that the relative expressions of MCP-1, IL-1β and TNF-α genes were highest in the obese group, which was in line with previous research that obesity poses a risk of inflammatory cytokines expression (Boutens & Stienstra, 2016; Remmerie, Martens & Scott, 2020; Cheng, 2016).