The effects of HNK on CFLAR-JNK pathway observed in this work are consistent with the previous reports [16, 27], and according to the previous research in the laboratory, the CFLAR-JNK pathway is closely related to the NASH [28], reflecting that HNK can inhibit the phosphorylation of JNK via activating CFLAR, ultimately alleviating the symptoms of NASH. Here, MAPK8 is linked to metabolic dysfunction-associated steatohepatitis.