In order to understand the molecular mechanism by which MaTAR25 imparts its function, we used ChIRP-seq and RNA-seq to identify and validate the Tns1 gene as a direct downstream target of MaTAR25. MaTAR25 positively regulates the expression of Tns1 in mammary cancer cells through binding to its DNA sequence in trans. Tns1 has been shown to localize to focal adhesions and fibrilla adhesions, and to assist in mediating signaling between the extracellular matrix and the actin cytoskeleton to impact cell movement and proliferation36,37. Here, TNS1 is linked to breast cancer.