Here, we compared mRNA expression of NANOG and SOX2 between tumor cells co-cultured with or without CAFs and found decreased mRNA expression of SOX2 in tumor cells co-cultured with CAFs from LK1108 (Fig. 4), but in the other two cell lines, a tendency to increased expression of SOX2 and NANOG was found. The gene discussed is NANOG; the disease is neoplasm.