Hence, based on the knowledge that these two populations are interconnected with other ones, such as mesenchymal stem cells and immune cells, future efforts will focus on the evaluation of a more complex in vivo system, by which we could clarify the potential role of ANXA1 as a prognostic/diagnostic soluble factor, even EVs captured, in the PC model. The gene discussed is ANXA1; the disease is pachyonychia congenita.