In order to investigate the role of ANXA1 on EVs-dependent metastatic potential of PC cells, we studied the paracrine effects of EVs derived by WT and ANXA1 KO MIA PaCa-2 cells on BJ fibroblasts, one of the cellular components of the tumor microenvironment [37,38]. This evidence concerns the gene ANXA1 and pachyonychia congenita.