The tissue localizations of tumor-infiltrating lymphocyte (TIL) subpopulations; CD3+ T cells, CD8+ T cells, CD3+FOXP3+ T cells (regulatory T cells, Tregs), and CD20+ B cells in tumor cell nest and surrounding stroma were profiled and quantified by automated quantitative pathology imaging system in primary and metastatic tumors. This evidence concerns the gene FOXP3 and neoplasm.