As with the obese IL-10fl/fl Foxp3-Cre+ mice, we were concerned that the phenotype we observed in the obese Blimp-1fl/fl Foxp3-Cre+ mice was due to colitis, as Blimp-1 expression in Tregs and local IL-10 production have been associated with suppressing intestinal inflammation (24, 27). This evidence concerns the gene IL10 and colitis.