Compared with cells transfected with si‐FXYD3, the expression of let‐7i and FXYD3 was enhanced in cells treated with si‐FXYD3 + EV‐mimic‐NC, the expression of KDM3A and DCLK1 was decreased, whereas the proliferation and invasion of lung cancer cells were inhibited, and the apoptosis was promoted. The gene discussed is DCLK1; the disease is lung cancer.