To further determine that DCLK1 regulates the lung cancer cell proliferation, migration and invasion through FXYD3, lung cancer cells were transfected with oe‐DCLK1 + oe‐FXYD3 and the results illustrated that the overexpression of FXYD3 led to increased FXYD3 expression but inhibited proliferation, migration and invasion abilities of lung cancer cells; however, these changes could be reversed by the addition of oe‐DCLK1 (Figure 5E‐G). This evidence concerns the gene DCLK1 and lung cancer.