The specificity of DCLK1‐42 mAb and DCLK1‐87 mAb in NCM460, HCT116 and colorectal cancer tissues has been previously confirmed, and a high degree of overlap was observed between DCLK1 and microtubule protein expression, indicating that both DCLK1‐42 mAb and DCLK1‐87 mAb recognized DCLK1 in the cytoplasm.25 The gene discussed is DCLK1; the disease is colorectal cancer.