At 48 h after transfection, cell apoptosis, proliferation, invasion, and migration were examined, indicating that, in contrast to the group miR-27b-OE, the recovery of EN2 contributed to the reversal of the survival actions, namely pro-apoptosis of miR-27b-3p, in which the ability of proliferation of BC cells was significantly recovered after EN2 was up-regulated (Figure 5A,D,G). The gene discussed is EN2; the disease is breast cancer.