EN2 and neoplasm: Additionally, a pathological study showed that compared with the group miR-NC+EN2-NC, miR-27b-3p up-regulation significantly alleviated histological heterogeneity of xenograft tumours and arranged the structure, hierarchy, and polar direction of cancer cells to be more regular, while EN2 re-introduction (the group miR-27b-OE+EN2-OE) reversed the above finding (Figure 6C).