Immunohistochemistry indicated that compared with the group miR-NC+EN2-NC, miR-27b-3p up-regulation significantly down-regulated the expression of EN2 protein in xenograft tumours, while EN2 re-introduction (the group miR-27b-OE+EN2-OE) reversed the inhibitory effects of miR-27b-3p on EN2 (Figure 6D). Here, EN2 is linked to neoplasm.