KMT2A and colonic neoplasm: To investigate the essential role of Mll1 in the stemness of human colon tumors, we established stable, doxycycline-inducible shMLL1 single-cell clones of the human colon adenocarcinoma cell lines Ls174T and DLD1, which exhibited a strong reduction of MLL1 (Supplementary Fig. 4a–d).