Moreover, an increased amount of purified antibodies corresponded to an increased reaction with antigen non-coated polystyrene plate, and the non-specific reaction was correlated with collagen type I (r = 0.91; p < 0.001), LPS O3 (r = 0.89; p < 0.001) Lys-GalA (r = 0.67; p = 0.009) and Thr-GalA (r = 0.96; p < 0.001), indicating that purified antibodies contain fractions of low specific characteristics for autoimmune diseases. This evidence concerns the gene GLA and autoimmune disease.