We tested this hypothesis in the immune cells by overexpressing miRNA mimics (miR-181a/b/c/d) in skewed macrophages and quantifying the expression levels of OPN in vitro and in glioma cells by establishing stably overexpressing miR-181a/b/c/d cell lines and quantifying the OPN expression in vitro. This evidence concerns the gene SPP1 and central nervous system cancer.