Similarly, Pena et al. found significant reductions in pro-inflammatory IFN-γ and TNF-α in the spleen of probiotic-treated mice using a mixture of Lactobacillus paracasei and Lactobacillus reuteri, which resulted in lessening the severity of colitis in IL-10-deficient mice infected with Helicobacter hepaticus [39]. This evidence concerns the gene TNF and colitis.